TY - JOUR ID - 196615 TI - Comparison between the field plants and their micropropagated plants of four Mentha spp. using molecular markers and phytochemical analyses JO - Egyptian Journal of Chemistry JA - EJCHEM LA - en SN - 0449-2285 AU - ahmed, suzan mosaad AU - Mohamed, Shereen Abu El-Maaty AU - El-Sadat, Neveen Hosney AU - Youssef, Sawsan Samy AD - Egyptian Drug Authority, Giza, Egypt. AD - Department of Genetics, Faculty of Agriculture, Cairo University, Egypt. AD - Genetics Department and Genetic Engineering Research Center, Faculty of Agriculture, Cairo University, Giza, Egypt. Y1 - 2022 PY - 2022 VL - 65 IS - 3 SP - 605 EP - 617 KW - Mentha KW - micropropagation KW - Phytochemical KW - SRAP KW - ISSR KW - Phylogeny DO - 10.21608/ejchem.2021.97034.4541 N2 - The efficient micropropagation procedures among four Mentha species (M. longifolia, M. spicata, M. viridis, and M. piperita) were established. In addition, the shoots were produced in 3.0 mg/l BA. M. longifolia had the highest percentage of regeneration (70%), whereas M. piperita recorded that the lowest value (47%). The micropropagated plants and field plants were subjected to phytochemical analyses. The gas chromatography and spectrophotometer results indicated that the active ingredients were changed in the regenerated plants. Nevertheless, the results of molecular markers showed that the thirteen SRAP primer combinations had 80 scorable bands; of them, 58 were polymorphic (72%). Nine ISSR markers showed a total of 60 scorable bands; of them, 44 were polymorphic (73%). Twelve out of the 102 polymorphic SRAP and ISSR markers were found to be genotype unique specific markers. UR - https://ejchem.journals.ekb.eg/article_196615.html L1 - https://ejchem.journals.ekb.eg/article_196615_9a25ba8aac53ca61947f9f4f56882bcf.pdf ER -