Chemical Investigation of Flavonoid, Phenolic acids Composition and Antioxidant activity of Tagetes erecta Flowers

Youssef, Hanim A; Ali, Safaa M; Sanad, Mostafa I; Dawood, Dawood H

doi:10.21608/ejchem.2019.19839.2197

Chemical Investigation of Flavonoid, Phenolic acids Composition and Antioxidant activity of Tagetes erecta Flowers

Document Type : Original Article

Authors

¹ Department of agriculture chemistry, faculty of agriculture, Mansoura university

² Department of agriculture chemistry, faculty of agriculture, Mansoura univeristy

³ Department of agriculture chemistry, faculty of agriculture,Mansoura univeristy

10.21608/ejchem.2019.19839.2197

Abstract

This study was geared towards discovering the chemical profile and in vitro antioxidant activity of methanol (MeOH) extract and its ethyl acetate (EtOAc) fraction of Tagetes erecta orange flowers. A total of 29 known chemical compounds including phenolic acids and flavonoid compounds were identified and quantified using high performance liquid chromatography (HPLC) technique. The identification was carried out in EtOAc fraction for the first time. Pyrogallol and vanillic acid were highly abundant phenolic acids in flowers methanolic extract and its EtOAc fraction amounted 37.57 and 3.34mg/g dry extract, respectively. On the other hand, apig-6-rhamnose-8-glucose and hesperidin were found to be the major flavonoid compounds detected in MeOH extract amounted 18.34 and 0.66mg/g dry extract, respectively. Furthermore, results asserted that the tested samples were more efficient in the three applied antioxidant methods compared with standard antioxidant references (Ascorbic acid and BHT), especially EtOAc fraction which displayed the most activity against 1, 1-di phenyl-2-picrylhydrazyl (DPPH) radical with IC50 value  32.5± 0.12 µg/ml. These finding highlighted the use of Tagetes erecta flowers as a promising source of alternative bioactive compounds for both industrial and medicinal uses.

Keywords