Anticancer Activity of Propolis Extract-ZnO Nanocomposite Against Human Lung Carcinoma Cell Line

Ismail, Naglaa M.; Korraa, Soheir; Sroor, Amany T.; Qotb, Amira Abdel Rehim

doi:10.21608/ejchem.2023.230583.8461

Anticancer Activity of Propolis Extract-ZnO Nanocomposite Against Human Lung Carcinoma Cell Line

Document Type : Original Article

Authors

¹ Physics department- Faculty of Women for Arts, Science and Education- Ain Shams University, Egypt

² Radiation Health Department- National Center for Radiation Research and Technology – Atomic Energy Authority, Egypt

10.21608/ejchem.2023.230583.8461

Abstract

This study aimed to evaluate the synergistic effect of propolis extract-zinc oxide (EEP-ZnO) nanocomposite against human lung carcinoma cell line. EEP-ZnO nanocomposite was formulated by the combination of Zinc Oxide nanoparticles (ZnO NPs) with ethanolic propolis extract (EEP) that was characterized by X-ray diffraction (XRD), Transmission electron microscopy (TEM) and Fourier transform infrared spectroscopy (FT-IR). The MTT assay and Annexin V-PI apoptosis assay were used for cytotoxicity evaluation of prepared EEP-ZnO nanocomposite against human lung carcinoma cell line (A549) and human lung normal cell line (WI-38). Results showed that the propolis extract- zinc oxide nanocomposite significantly decreased cell viability and increased cell apoptosis, as compared to EEP and ZnO NPs alone. It was also observed that EEP, ZnO NPs, and EEP-ZnO nanocomposite are exhibited a less toxic effect on WI-38 comparable to A549.

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