Novel Bacterial Isolate For Amylase Production, Characterization And Application On Textile Using Saffron Natural Dye

Document Type : Original Article

Authors

1 Natural and microbial products Chemistry department, Drug and Pharmaceutical Division, National research Center

2 dyeing and printing department, Textile research division, National research center

Abstract

The screening experiments from soil samples led to the isolation of some amylase-producing bacterial isolates. The bacterial isolate showed maximum amylase production was selected for further physiological and biochemical investigations. The results showed that the maximum activity (125u/ml)was obtained by using a fermentation medium consisting of (g/l) starch,10 peptone 10 yeast extract 5, ammonium sulphate 5, magnesium sulphate 0.25, CaCl2 0.25 between 80 1ml at pH 5.6,fermentation time 48 h at 300C.The obtained amylase underwent partial purification using ammonium sulphate at conc.60wt%.The purified enzyme was applied to wool fibers dyed with saffron natural dye. The phylogenic analysis of the isolated strain appeared to agree with the Bacillus mycoides. The results obtained exhibited higher color strength for the treated samples compared to the untreated fibers

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History

  • Receive Date: 10 August 2023
  • Revise Date: 16 October 2023
  • Accept Date: 29 October 2023

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