Citharexylum spinosum promotes antioxidant, anti-inflammatory, and anti-acetylcholinesterase activities

Saad, Safaa A.; Abd-Alla, Howaida I.; Aly, Hanan F.; Shalaby, Nagwa M. M.; Afify, Abd El-Moneim M. R.; Ali, Hanaa F. M.; Abdel-Moein, Nadia M.

doi:10.21608/ejchem.2023.235755.8594

Citharexylum spinosum promotes antioxidant, anti-inflammatory, and anti-acetylcholinesterase activities

Document Type : Original Article

Authors

¹ Natural Compounds Chemistry Department, National Research Centre, Dokki 12622, Giza, Egypt

² Therapeutic Chemistry Department, National Research Centre, Dokki 12622, Giza, Egypt

³ Biochemistry Department, Faculty of Agriculture, Cairo University, Egypt

10.21608/ejchem.2023.235755.8594

Abstract

Citharexylum spinosum leaves extract; n-hexane (n-Hex), n-hexane:benzene (n-Hex:Bz), chloroform (CHCl3), ethyl acetate (EtoAc), n-butanol (n-BuOH) and ethanol:H2O50% (Eth:H2O 50%) were evaluated for their anti-acetylcholinesterase, antioxidant and anti-inflammatory activities. The highest anti-acetylcholinesterase activity was obtained by n-BuOH extract followed by n-Hex:Bz and Eth:H2O 50% (IC50; 0.60, 0.80 and 1.86 µg/ml, respectively). The antioxidant results showed that, n-BuOH exhibited the highest DPPH● scavenging activity (85.91, 87.32, 56.33 and 39.43%) at different concentrations (1-100µg/µl), and 391.71±3.2μg/ml for H2O2 antioxidant method. While Eth:H2O 50% extract exhibited the highest antioxidant by reducing power method, FRAP and ABTS● scavenging activity (87.94%, 1382.29µmol of Fe2+/g sample and 20.01mmol Trolox/g sample, respectively). The highest anti-inflammatory effect obtained by n-BuOH extract followed by Eth:H2O 50% (IC50 74.50 and 109.80μg/ml, respectively). The screening of bimolecular components was carried out for n-BuOH and Eth:H2O 50% extracts by using LC-MS/MS. The LC-MS/MS profile showed the presence of organic acids; chlorogenic, gallic, caffeic, coumaric, ellagic, cinnamic, 3.4-dihydroxybenzoic, methyl gallate, syringic and ferulic) and flavonoids (rutin, vanillin, naringenin, querectin and luteolin). The current study aimed to for the first time to evaluate the anti-cholinesterase activity of C. spinosum leaves besides, the determination of the antioxidant and anti-inflammatory activities by different methods.

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