Enhancement of Diazinon Degradation, Isolation of Organophosphorus Hydrolase and Chemical Analysis of Metabolites

Abosereih, Nivien. A.; El-sayed, Ghada. M.; Ibrahim, S. A.; Abd El-Razik, A. B.; Hammad, M.A.; Hafez, F. M.

doi:10.21608/ejchem.2019.11689.1748

Enhancement of Diazinon Degradation, Isolation of Organophosphorus Hydrolase and Chemical Analysis of Metabolites

Document Type : Original Article

Authors

¹ Department of Microbial Genetics, National Research Centre, 33 El-Bohouth St., Dokki, Giza 12622, Egypt.

² Department of Genetic, Faculty of Agriculture, Ain Shams University, Cairo, Egypt.

³ Department Plant Protections, Faculty of Agriculture, Ain Shams University, Cairo, Egypt

10.21608/ejchem.2019.11689.1748

Abstract

In this study, diazinon high degradation was achieved by using Ethyl methane sulfonate (EMS) as a chemical mutagen, 38.19% of diazinon was degradedin the minimal media as compared to 1.83% in control media after 5 days.Due to the first step of ethylmethane sulphonate (EMS) chemical treatment, diazinon degradation increased to 62.19% which was subsequently increased to 86.21% after the EMS treatment second step. Analysis of organophosphorus hydrolaseprotein involved in diazinon hydrolysis revealed that itis a member of the MBL-fold metallo hydrolase superfamily and has beta lactamase fold.2-Iso-4-methyl-6- hydroxypyrimidine (IMHP) was detected as the main degradation product of diazinon through GC/MS analysis after 5days of incubation and further metabolized to unknown polar metabolites that weredetected using GC/MS after 10 days.

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