Document Type : Original Article
Authors
1
Department of Biochemistry and Molecular Biology, Theodor Bilharz Research Institute, Kornaish El-Nile St., 12411, Giza, Egypt
2
Department of Biochemistry and Molecular Biology, Theodor Bilharz Research Institute, Kornaish El-Nile St., 12411, Giza, Egypt.
3
Department of Biochemistry, Faculty of Science, Ain Shams University, Cairo, Egypt.
4
Department of Medicinal Chemistry, Theodor Bilharz Research Institute, Kornaish El-Nile St., 12411, Giza, Egypt.
Abstract
Spirulina platensis (S. platensis) is a blue-green microalga that belongs to the cyanobacteria family. The goal of this research was to investigate the anticancer activity of encapsulation Spirulina platensis algae with Chitosan-Alginate polyelectrolyte nanoparticles on HepG-2, Caco-2, and Vero cell lines and profile its chemical components . S.platensis was extracted using 90% MeOH as a crude extract which was fractionated using different organic solvents. While Nanoencapsulation of 90% MeOH ext. and pet-ether fr. by Chitosan-Alginate polyelectrolyte nanoparticles were prepared by modified ionic gelation method. The chemical composition of 90% MeOH ext. was identified using the GC-MS technique. The 90% MeOH ext. of S. platensis showed high toxicity on HepG-2 and Caco-2 cells, more than the pet-ether fraction. While they were safe on Vero normal cells. Furthermore, nanoencapsulation of the 90%MeOH ext. and pet-ether fr. increased the cytotoxicity against HepG-2 and Caco-2 cells. Moreover, The effect of 90%MeOH ext. and pet-ether fr. NPs against HepG-2 and Caco-2 resulted in a significant increase in the relative expression of the Caspase-3 enzyme and a decrease in the expression of the TNF-α gene and COX-2 enzyme more than the non-encapsulated form. The GC-MS analysis of the silylated 90 % MeOH ext. revealed the presence of 57 bioactive compounds including, alcohols, terpenes, organic acids, and others.
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