Carpaine: Multitarget docking for its Antiproliferative Potential on CHO-k1 Human Ovarian Carcinoma

Document Type : Original Article

Authors

1 School of Life and Medical Sciences, University of Hertfordshire hosted by Global Academic Foundation,New Administrative Capitol 11578, Cairo, Egypt.

2 Chemistry of Medicinal Plants Department, National Research Centre, 33 El Bohouthst. (Former El Tahrir St.), Dokki, Giza P.O.12622, Egypt.

Abstract

Ovarian cancer (OC) accounts as a common critical malignant tumor among female disease. Among numerous drug resources, botanical compounds identified and isolated from plants have unique advantages due to their potentials as chemotherapeutic drugs for cancer treatment. This work reports the extraction of carpaine from the leaves of Carica papaya using microwave-assisted extraction (MAE). Quantitative analysis of carpaine in the ethanol extract was conducted using HPLC with UV detection. The antiproliferative activity of extracted carpaine was investigated on CHO-K1 cell line (ovarian carcinoma) by MTT assay. Molecular modeling was employed to check the binding mode of carpaine with the 20S proteasome enzyme. Carpaine was detected in the alkaloid residue at 304 nm with retention time 36.50 min and the HPLC analytical methodology for standard curve was developed and validated to quantify carpaine as 0.29% DW of the dried leaves of Carica papaya. It was highly active against the human ovarian cancer cell line, CHO-K1, with IC50 value of 28.40±3.2 μg/ml compared to vinblastine sulfate, the reference standard, which gave IC50 value of 9.65±0.74 μg/ml. Molecular study emphasized the influential carpaine fitting in the binding site of 20S proteasome enzyme suggesting it is the prospective key player for carpaine’s observed anti-ovarian cancer activity. These results suggest important guidance for the potential pathway of carpaine as an effective Carica papaya’s extract component for the prevention or amelioration of cancer and consequently, highly expected to be utilized as functional foods contributing to the preservation and promotion of human health.

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Egyptian Journal of Chemistry
Volume 66, Issue 13 - Serial Number 13
Special Issue: Applied Chemistry for Greener Life and Sustainability
December 2023
Pages 755-761

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History

  • Receive Date: 02 February 2023
  • Revise Date: 12 April 2023
  • Accept Date: 02 May 2023

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