Biochemical and Molecular Characterization of Some Rice Accessions Tolerated To Salt Stress

Document Type : Original Article

Author

Genetics & cytology Department, Biotechnology Research Institute, National Research Centre, 33 El Buhouth ST, Postal code 12622, Dokki, Cairo, Egypt

Abstract

Rice is considered one of the most important food crops, and it ranks third in terms of strategic nutritional importance after wheat and maize. Given that this strategic crop is sensitive to salt stress, especially in the areas designated for rice cultivation in the valley and delta regions, it was necessary for researchers to develop new lines with good tolerance in addition to, their high yielding through using the original rice cultivar (Giza 178 and its 7 mutants derided from it). These seven mutants were derived by five gamma rays doses; 100, 200, 300, 400 and 500 and characterized with highly rank of stable. Indeed, this investigation succeeded in developing and eliciting a number of promising rice accessions that are tolerant to salt stress and high output using various doses of gamma rays while, highlighting the biochemical, physiological and genetic evidence of this tolerance under Egyptian conditions. Based on this, these promising new lines represent a major scientific leap in the field of breeding and improving rice tolerance to face the risk of soil salinity, which seemed to be increasing in the past few years due to water scarcity needed for irrigation and the fact that Egypt is in an area of water stress. The final results confirmed that the highest rice mutants recorded highly rank of yield components and the other attributes under salinity treatment compared to the standard experiment were mutant 2, followed by mutant 7 and then followed by mutant 3, respectively. Further, the five ISSR primers discovered 2 unique markers considering that these fragments are the taxonomic basis for salt stress tolerance in the five new rice mutants compared to the original variety and this is the biggest progress in this investigation.

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Volume 66, Issue 13 - Serial Number 13
Special Issue: Applied Chemistry for Greener Life and Sustainability
December 2023
Pages 881-891
  • Receive Date: 09 February 2023
  • Revise Date: 26 April 2023
  • Accept Date: 30 April 2023