Chemical Investigation of Phenolic Profiles and Antioxidant Activity of Chrysanthemum Morifolium

Document Type : Original Article

Authors

1 Department of Agricultural Chemistry, Faculty of Agriculture, Mansoura University, Mansoura, Egypt

2 Department of Agricultural Chemistry, Faculty of Agriculture, Mansoura university, Mansoura, Egypt

Abstract

This study is focused on determining the phenolic profile and antioxidant activity of Chrysanthemum morifolium using, methanol (MeOH) and ethyl acetate (EtOAc) as solvents. The in vitro antioxidant activity was studied adopting three different tests: reducing power assay, Phosphomolybdenum antioxidative power assay and inhibition of free radical 1,1-di phenyl-2-picryl hydrazyl (DPPH). Total phenolic content (TPC), using Folin Ciocalteu reagent ranged from 27.36±0.7 to105.72±0.64 mg gallic acid equivalent (mgGAE)/g D.W. While, total flavonoid content (TFC) ranged from 3.76±0.41 to 37.75±1.8 mg quercetin equivalent (mgQE)/g D.W. A total of 29 phenolics including 15 phenolic acids and 14 flavonoids, were characterized and quantified using high performance liquid chromatography (HPLC). Ellagic acid was the prevalent phenolic in green aerial parts MeOH extract and its EtOAc fraction (23.11and 35.63 mg/g, dry extract respectively). Hesperidin was the dominant flavonoid in flowers MeOH extract amounted 43.23mg/g, dry extract. Flowers EtOAc fraction was the most efficient in all applied antioxidant methods. While, Green aerial parts methanolic extract was more dynamic than its EtOAc fraction, except in reducing power assay with EC50 value 2020.5±27.97 µg/mL. The results of this study implied that the analyzed samples were important natural sources for bioactive compounds with potential antioxidant activity.

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History

  • Receive Date: 18 February 2022
  • Revise Date: 11 March 2022
  • Accept Date: 14 February 2023

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