Optimization and Scaling-up of Cyclodextrin Glucosyltransferase Production by the Local Isolate Bacillus paramycoides MZ005634

Saleh, AM; Zaki, RA; Gomaa, SK; Elrefai, HA; Shafei, MS; Abd-Elsalam, IS; Elrefaey, AH

doi:10.21608/ejchem.2023.176162.7219

Optimization and Scaling-up of Cyclodextrin Glucosyltransferase Production by the Local Isolate Bacillus paramycoides MZ005634

Document Type : Original Article

Authors

¹ Chemistry of Natural and Microbial Products Department, National Research Centre, Dokki, 12622 Giza, Egypt

² Department of Industrial Engineering, College of Engineering, American University of Sharjah, United Arab Emirates.

10.21608/ejchem.2023.176162.7219

Abstract

Cyclodextrin glycosyltransferase is an important industrial enzyme that converts starch in to cyclodextrins (α-, β-, and ɣ-CDs). In this study our aim was to optimize the activity of produced CGTase for the production of β-CD from a local bacterial isolate and up-scaling the fermentation process. A terrestrial Bacillus paramycoides was isolated from soil obtained from sewage of Ismailia canal and identified by 16S rRNA analysis for the production of CGTase enzyme. Fractional factorial design (FFD) was applied to analyze the influence and describe the relationship between the tested variables: soluble starch, fermentation time, peptone, KH2PO4, inoculum size and pH. The results represent an improvement in CGTase activity of 1.10 folds when compared to that previously obtained using the basal medium under the same cultivation conditions. The generated model was found to be very adequate for CGTase activity (81.57% accuracy) as the experimental value was compared to the predicted value. Upon applying a series of batch fermentations with different agitation rate an increase in CGTase activity about 1.6 folds compared to that obtained using shaked flasks.

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