Enzymatic degradation of white bovine pickled shavings yielding industrial gelatin and collagen hydrolysate

Saber, Abdelmohsen; Elsayed, Nabil Hussein; El-Abd, Mostafa Abd El-Hameed; Hassan, Amira Aly; Abo Alkasem, Mohamed Ibrahim; Marzouk, Mohammed Sonhi; Elsayed, Hamed

doi:10.21608/ejchem.2022.160832.7007

Enzymatic degradation of white bovine pickled shavings yielding industrial gelatin and collagen hydrolysate

Document Type : Original Article

Authors

¹ Chemistry of Natural and Microbial Products Dept., Pharmaceutical and Drug Industries Research Div.National Research Centre, Dokki, Giza, Egypt P.O. Box: 12622

² NRC, cairo, Egypt

³ Chemistry of Natural Products, NRC, Giza, Egypt

⁴ Chemistry of Natural and Microbial Products Department, Pharmaceutical Industries Research Institute, National Research Centre, Dokki, Giza, Egypt.

⁵ Chemistry of natural and microbial products, Pharmaceutical research division. National Research Centre, Giza, Egypt

⁶ chemistry of tanning materials, chemical industry division. NRC

⁷ Chemistry of tanning materials and leather technology department, National Research center

10.21608/ejchem.2022.160832.7007

Abstract

Six fungal species (T 1-6) and twelve isolates from bacterial fish wastes (BFW (1-7) and white pickled shavings wastes WPSW (1-5), excluded from Egyptian leather industry were examined for its utilization as a source for industrial gelatin and collagen hydrolysate. The conversion of the native/pretreated WPSW was conducted through protease blends in simultaneous processes. All the conducted studies confirmed the superiority and efficiency of the bacterial isolates in 2-d- shaken cultures at 37 ℃ over all the tested fungal species under the proper conditions for proteases, especially collagenases productivity. The three bacterial isolates BFW5, WPSW3 and WPSW5 proved their prevalence as potent collagenase producers, which stimulates good gelatin formation through the controlled enzymatic hydrolysis of the two WPS (native/pretreated) specimens, while at the same conditions, the isolates BFW7, WPSW3 & WPSW5 identified as (Citrobacter freundii) were nominated as potent hydrolysate producers. BFW5 identified as (Bacilus cirus) crude enzyme mixture surpassed all the others and afforded 42.5% lab scale gelatin formation efficiency, while the recorded hydrolysate % was only 5.97%. On the other hand, the bacterial isolate WPSW5 afforded the highest hydrolysate estimate (7.12%).

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