A simple and accurate spectrophotometric method for simultaneous determination was used in this research of 3,3′,5-triidothyronine and thyroxine in human serum samples. The method created on the zero – crossing calculation for second and third derivative spectrophotometry. The calibration graphs are in the concentration spectrum, linear of 1.0 – 17.0 µgmL-1 and 1-18 µgmL-1 for T3 and T4 successively. The recoveries range from 93.78 – 102.68 % for T3 and 93.47 – 103.25 % for T4 with relative standard deviation less than 4.28% and 4.50% in all in stance for T3 and T4 respectively.
Fakhre, N. A., Riasheed, C. M., & Ali, D. K. (2021). Second and Third Derivative Spectrophotometric Determination of Liothyronine and Thyroxine in Human Serum. Egyptian Journal of Chemistry, 64(11), 6385-6397. doi: 10.21608/ejchem.2021.77986.3811
MLA
Nabil A. Fakhre; Chnar M. Riasheed; Dashty Kh. Ali. "Second and Third Derivative Spectrophotometric Determination of Liothyronine and Thyroxine in Human Serum". Egyptian Journal of Chemistry, 64, 11, 2021, 6385-6397. doi: 10.21608/ejchem.2021.77986.3811
HARVARD
Fakhre, N. A., Riasheed, C. M., Ali, D. K. (2021). 'Second and Third Derivative Spectrophotometric Determination of Liothyronine and Thyroxine in Human Serum', Egyptian Journal of Chemistry, 64(11), pp. 6385-6397. doi: 10.21608/ejchem.2021.77986.3811
VANCOUVER
Fakhre, N. A., Riasheed, C. M., Ali, D. K. Second and Third Derivative Spectrophotometric Determination of Liothyronine and Thyroxine in Human Serum. Egyptian Journal of Chemistry, 2021; 64(11): 6385-6397. doi: 10.21608/ejchem.2021.77986.3811
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